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All the shotgun and DIA raw data was directly analyzed by MaxQuant [15] and searched against the human canonical UniProtKB/Swiss-Prot database (downloaded February 2018, 20,258 entries). Oxidation at methionine was set as variable modification, whereas carbamidomethylation at cysteine was set as a fixed modification. Up to two missed cleavages were allowed. The mixed A2780LH sample was searched following standard SILAC setting. Other parameters are kept as default in MaxQuant. Both peptide and protein level were controlled at 1% FDR [16]. The match between run function was disabled and the second peptide search function was enabled (as default).

Komoot is a navigation and route planning app that enables users to create and follow routes based on riding type and ability.\nDesigned to help users explore new places and discover new routes, as well as provide ride recommendations through its \u2018community\u2019 of riders, Komoot is one of the most popular route planning apps on the market and has many functions that aim to make route planning an easier experience.\nAs cycling has shifted more towards adventure riding, exploration and gravel riding, Komoot has proven popular with cyclists thanks to its easy interface and robust route planning algorithm. It is included in BikeRadar\u2019s list of the best cycling apps and is compatible with the best bike computers.\nWhat is Komoot?\n\n On Komoot\u2019s website, you can filter Tours by duration and difficulty. Komoot \nKomoot is a route planning and navigation app. It allows you to plan routes on your mobile or computer and will generate routes based on riding type, surface and rider ability.\nUsing a start and endpoint, Komoot will tell you the distance, elevation profile, road surface, fitness level required, difficulty and estimated time needed to complete the route.\nYou can use these routes to navigate via your mobile phone, GPS cycling computer or Garmin cycling watch without an internet connection.\nIf you have access to an internet connection or mobile data, Komoot also allows you to reroute when you are out on a ride if you decide you want to head somewhere different or just change course.\nThe app also has a strong community focus. This means you can discover places other users like to ride or visit (\u2018Highlights\u2019 in Komoot parlance), such as particular climbs, cafes or points of interest and integrate these into your ride. You can use routes they have planned too.\nKomoot is available to use on desktop via the Komoot website. You also download Komoot for iOS or Komoot for Android.\nHow does Komoot work?\n\n Komoot enables you to filter Tours by location and riding discipline. Komoot\nKomoot uses the open-source OpenStreetMap database for its base mapping but then uses its own algorithms to establish the best route depending on activity type, selected waypoints and points of interest.\nKomoot provides map overlays including an Open Cycle Map that shows national cycleways and a Google satellite map. You can also upload GPX or FIT route files to Komoot.\nOnce a route has been selected, Komoot will give you onscreen and audible navigation in an offline mode to save the battery on your device. You can also send routes created on Komoot to your compatible cycling computer for navigation.\nWhen riding, the Komoot app will tell you speed, distance travelled, distance remaining and let you alter the route on the fly.\nWhen you have completed your ride you can upload it to your Komoot profile and add photos.\nKomoot will also feature local highlights, as suggested by users and Komoot ambassadors.\nKomoot can be used on a computer (PC or Mac), iPhone, Android phone, Garmin, Wahoo and most wearable devices, such as Apple Watch and Android Wear.\nHow do I get started on Komoot?\n\n The Komoot app is available on Garmin devices. Komoot\nTo start using Komoot you will need to set up an account.\nOn a Mac or PC, you can do this via Komoot\u2019s website. On a smartphone, you can do it via the Komoot app, which you can download from the Apple App Store or Google Play.\nYou can start using Komoot for free straight away, planning routes and navigation. You can also pay to download maps for offline use.\nKomoot also offers a Premium account that is aimed at adventurers and those looking to complete multi-day trips \u2013 it also offers discounts on products.\n\n Navigate with Komoot from your Garmin watch. Komoot\nOnce you are set up with a Komoot account and the app, you can integrate Komoot with your Garmin or Wahoo to send routes to the device.\nKomoot has a proprietary app for Garmin that can be downloaded through the Garmin Connect IQ store.\nFor Wahoo, all you need to do is go to your Wahoo app, head to connections and login into your Komoot account. You will then be able to access routes and sync your rides to the Komoot app.\n\n \nKomoot vs Strava\nThe main difference between Komoot and Strava is that Komoot allows you to plan routes using the free version of the app whereas Strava now requires you to subscribe to access its route planning service.\u00a0\nWith its QOM\/KOMs, segment leaderboards and performance analytic tools (for paid subscribers), Strava focuses more on performance.\u00a0\nOur Komoot vs Strava guide examines the differences in more detail.\u00a0\n\n\n \n Komoot vs RideWithGPS\n\n RideWithGPS permits more granular route planning. Ride with GPS\nRideWithGPS is another popular route-planning app best suited to those who like to spend hours carefully planning rides.\nCompared to Komoot, it\u2019s more user-friendly if you like to create routes waypoint-by-waypoint, particularly if they cross unmapped trails. The ability to draw lines freehand, rather than follow defined routes, helps here (Komoot offers similar functionality, but its implementation is a bit clunky).\nEditing existing routes \u2013 either made in the app or based on imported GPX files \u2013 is also easier in RideWithGPS.\nRideWithGPS offers 11 map layers as standard in its free plan. Komoot only offers four, with three additional layers available as part of a premium subscription.\nRideWithGPS features none of the crowd-sourced routing automation that defines Komoot as an app \u2013 while it offers heatmaps, these tell you little about conditions on the ground.\nWhere Komoot provides helpful insights in the form of highlights, RideWithGPS includes no such information. Any research must be done off-app.\nA basic RideWithGPS account is free to use, with two paid tiers offering additional functionality.\nHow do you plan a route using Komoot?\n\n When you plan a route in Komoot, it will show you waypoints and highlights along the way. Komoot\nTo create a route on Komoot go to the Route Planner tab on the website or in the mobile app.\nThen search for a destination and a starting point and the Komoot algorithm will calculate the best route either as an A to B journey or round trip.\nYou can select your chosen riding discipline (road, gravel or mountain bike for instance) and Komoot will adapt the route to best suit whatever bike you\u2019ll be riding.\nAdjusting fitness level on a sliding scale from Untrained to Pro will also affect how long Komoot says the route will take you.\nYou can add additional waypoints to your ride \u2013 for example, if you want to pass through a particular place or complete a certain climb \u2013 and Komoot will suggest the best route to include these.\nThe Komoot map also shows points of interest, amenities and highlights submitted by users (shown as red pins with bicycles on them). If you add these to your route, Komoot will adjust the route to include these too.\n\n Komoot warns you where might not be suitable for skinny tyres. Komoot\nKomoot will use its own mapping based on activity type to decide on a route, but you can plan a route using an \u201cOff-grid\u201d function that will let you ignore Komoot\u2019s network.\nThis won\u2019t always be useful \u2013 and might be better if you\u2019re using Komoot for running or hiking \u2013 but it could be good if you know a path or road exists or you have seen one on another map or satellite view.\nKomoot will show a breakdown of how long the route will take to complete, elevation, descent, its difficulty and the distance spent on each way type and surface.\nOnce you have decided on your route, with as many added features, waypoints and detours as you desire, you can save the route to your profile. It will then be available to use for navigation, send to a device, download as a GPX file or share with your riding buddy.\nDesktop vs app for route planning\n\n Some route planning tools, such as 3D maps, are only available on desktop. Komoot \nYou can plan routes on Komoot on either a mobile or desktop device, but it is probably worth considering using a desktop for in-depth planning because this gives you more room to see your route and it allows you to dive deeper into the functionality Komoot provides.\nCertain paid-only features such as Trail View and 3D Maps are available exclusively on desktop.\n\n Trail View is one of several map layers you can switch between. Komoot\nTrail View harnesses AI to match images of trails or roads uploaded by users to their location. Green dots indicate where these are on the desktop map. Clicking on one brings up a photo of the surface, enabling you to preview the terrain before you ride it.\nThe 3D view on the desktop route planner permits you to scroll in three dimensions through a landscape in the Komoot map or satellite layer.\nHowever, Komoot satellite map view is now available on the iOS and Android apps. Tap on the bottom right-hand corner of your phone screen to switch to satellite view to get a clearer idea of where you\u2019ll be riding.\nKomoot has also added Highlights warnings to the mobile app. These alert you if your tour includes a dangerous segment, such as a steep descent, or a national park where you may need to adhere to certain regulations.\nHow do you navigate using Komoot?\n\n Komoot is popular with bikepackers because you can plan multiple stages of your adventure. Chris McClean \/ Komoot\nKomoot can help you navigate in a number of ways, either by linking up to a cycling head unit or directly on your mobile phone.\nPhone navigation\n\n Mobile navigation while cycling is best with a phone mount. Komoot\nIf you are using a mobile phone, you simply select the route you wish to follow and hit the \u201cStart Navigation\u201d button.\nKomoot will then give you visual and audio turn-by-turn directions, so you can either listen through headphones or use a bike phone mount for onscreen directions.\nKomoot provides one free region for users that will provide navigation offline, but if you are venturing further afield you will need a data connection.\nAlternatively, you can buy maps. This is particularly useful if you want to save the battery life of your phone, save money on data when cycling abroad or explore places without phone signal.\nIf you want to change where you are going while riding you can simply select the location on the map and Komoot will reroute for you.\nCycling computer navigation\n\n Adjustments to a route in the app transfer to your head unit after syncing. Komoot \nOnce you have synced your Komoot app to your cycling computer, you will be able to follow any routes you have planned via your head unit.\nAny changes you make to the route via the app will be updated on your device, even as you go. You just need to replan and resync.\nHow do you upload your ride to Komoot?\nOnce you have completed your ride it will automatically sync to your Komoot profile as a private activity, or as Komoot calls it a \u201cTour\u201d.\nYou can then go into the Tour, name it, add photos and share it with other Komoot users. You can also select certain points on the ride and make it a highlight, playing your part in the Komoot community.\nOur list of 12 lesser-known UK cycling highlights has links to relevant Komoot routes to give you an idea of what sort of rides you can discover when using the app.\nWhat is the Komoot community?\n\n Dozens ride the Komoot Women\u2019s Torino-Nice rally. Bicycle Factory\nSince it was founded in 2006, Komoot has grown to over 20-million users worldwide.\nReferring to users as a community might seem like a stretch, but it begins to make sense when you consider that Komoot\u2019s power really comes through input from its users.\nAs already mentioned in this guide, the Highlights function on Komoot lets you find out about particular climbs, roads and points of interest wherever you\u2019re looking on the map.\nUsers are encouraged to add these highlights and to include pictures and descriptions to help other users find out more about an area they may not know, which is particularly useful for bikepacking.\nOther users can then add their own descriptions \u2013 almost like reviews \u2013 and pictures to these highlights, and can upvote or downvote the highlight too.\nKomoot also allows users to explore routes or Tours created by other users, seeing highlights and images from a ride as well as descriptions too, and use these for their own rides.\nKomoot may appoint a particular user as a Pioneer. Pioneers are users who find \u201cthe most beautiful Highlights\u201d and \u201cshare their expertise\u201d helping other users in the process.\nAs the number of users increases, Komoot should become a richer and richer resource for route planning.\nHow much does Komoot cost?\n\n Komoot Premium enables you to organise Tours into Collections. Komoot\nKomoot is free to use as soon as you set up an account, but there are various packages and in-app purchases you can make to enrich the experience.\nKomoot maps\nKomoot maps is the free version of the app. You can plan routes, upload rides and create highlights and use the social aspect of the app to comment on and like other users\u2019 activities. Navigation is also free but only with a data connection.\nYou can download maps of regions to navigate and reroute offline. It costs \u00a33.99 \/ $3.99 \/ \u20ac3.99 for a region and \u00a38.99 \/ $8.99 \/ \u20ac8.99 for a bundle of regions.\nYou can also pay \u00a329.99 \/ $29.99 \/ \u20ac29.99 to unlock maps for the whole world. This is a one-time purchase and lasts indefinitely.\nKomoot Premium\n\n You can purchase region maps in the free version of Komoot. Komoot\nKomoot has a premium subscription service that costs \u00a359.99 \/ \u20ac59.99 annually. There is no option to pay monthly.\nThe Premium subscription has a number of additional features which include:\nMulti-day planner that allows you to plan a complete bikepacking trip in one route and break it into daily segments\nOn-tour weather that gives you an up-to-date weather forecast for your trip\nSport-specific maps that overlay features such as mountain bike trails or cycling networks\nPersonal collections let you group routes and highlights into your own collections so you can find your favourite tours more easily\nDiscounts in 14 countries on products from 900 different brands\n\n Upgrading to Premium unlocks the multi-day planner, which is designed for tourers and bikepackers. Komoot\nThe Premium service includes maps and if you have already unlocked world maps you can get Premium at a discount price.\nCurrently, Komoot offers an insurance policy in its Premium package for users in Germany and Austria and plans to make this available in other countries soon.","image":"@type":"ImageObject","url":"https:\/\/images.immediate.co.uk\/production\/volatile\/sites\/21\/2021\/07\/405528-1910271000-aa2410-original-1635525560-89a3d61.jpg?quality=45&resize=768,574","width":768,"height":574,"headline":"Komoot: your complete guide","author":["@type":"Person","name":"Stan Portus"],"publisher":"@type":"Organization","name":"BikeRadar","url":"https:\/\/www.bikeradar.com","logo":"@type":"ImageObject","url":"https:\/\/images.immediate.co.uk\/production\/volatile\/sites\/21\/2019\/03\/cropped-White-Orange-da60b0b-04d8ff9.png?quality=90&resize=265,53","width":182,"height":60,"speakable":"@type":"SpeakableSpecification","xpath":["\/html\/head\/title","\/html\/head\/meta[@name='description']\/@content"],"url":"https:\/\/www.bikeradar.com\/advice\/buyers-guides\/guide-to-using-komoot\/","datePublished":"2023-02-06T12:00:00+00:00","dateModified":"2023-02-06T12:08:17+00:00"}] Komoot: your complete guide What Komoot is, how to use it and how it compares to Strava and RideWithGPS